I try to cultivate slime mold by regularly transferring small pieces of it in new plastic containers, hoping it will extend its plasmodial network all over the new dishes. However, not even 24 hours after the inoculation has been done, I’m puzzled to see only a small area of the dish visibly populated by the slime mold.
In the past couple of weeks, as I started working more and more with 2% agar substrates, I also got a better idea of what happened. I tilted the plate slightly in the light, only to see networks of transparent slime that show how far the Physarum has actually travelled. I’ve added filters on this particular picture —and the one above— to better show this:
How could better plasmodial formation be achieved?
Perhaps by checking on the slime mold more regularly than 24 hours increments. I’ll have to experiment by adding oat flakes as food sources at least twice a day — instead of once every other day or so — to see how the slime mold goes.